Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method

Cryopreservation of Rat Bone Marrow Derived Mesenchymal Stem Cells by Two Conventional and Open-Pulled Straw Vitrification Methods

Bone marrow (BM) is a complex tissue containing populations of progenitor and stem cells (1). One type, hematopoietic stem cells (HSCs), can renew circulating blood elements such as red blood cells, monocytes, platelets, granulocytes and lymphocytes. The other is mesenchymal stem cells (MSCs), which possess two important properties of long-term self renewal and differentiate into osteoblasts, c...

StemCell Keep™ Is Effective for Cryopreservation of Human Embryonic Stem Cells by Vitrification.

Safe and stable cryopreservation is critical for research involving human embryonic stem cells (hESCs). Dimethyl sulfoxide (DMSO) is a popular cryoprotective agent; however, its cytotoxicity cannot be ignored. Thus, there is a need for an alternate cryoprotectant. We reported previously that a novel cryopreservation reagent, StemCell Keep™ (SCK), was effective for cryopreserving human induced p...

StemCell KeepTM Is Effective for Cryopreservation of Human Embryonic Stem Cells by Vitrification

Bulk vitrification of human embryonic stem cells.

BACKGROUND The traditional vitrification method cannot keep up with the increased culture and propagation efficiency required to cryopreserve large quantities of vigorously proliferating human embryonic stem (HES) cells. In this study, we describe a newly invented vitrification carrier for cryopreserving large amount of HES cells and evaluate whether this bulk vitrification (BV) method is as ef...

Open Pulled Straw (OPS) Vitrification of Mus Musculus Morula and Blastocyst Survival in Two Common Cryopreservation Medias

The objectives of this study were to: (1) determine an optimal cryoprotectant of mice embryos; and (2) determine whether morula or blastocyst stage is ideal for vitrification in both medias. One experiment was performed using two different medias for vitrification with open pulled straws (OPS) with morulae and blastocysts. In the first protocol, we called V, embryos were exposed to 10% ethylene...